Unlock Target ProteinProtein Interactions: Pull-Down based Protein Analysis Service at MtoZ Biolabs

MtoZ Biolabs utilize GST Pull-Down kits, paired with a high-sensitivity UPLC-MS platform, to offer the most optimized one-stop solution for pull-down based protein analysis service. MS not only offers high sensitivity and specificity but also allows for the accurate identification of low-abundance and complex protein interaction networks. By applying MS to identify the proteins captured in the pull-down assay, it provides precise molecular-level information in a high-throughput context.

Pull-down based protein analysis for target protein discovery provides a key biological and biochemical basis for understanding biological processes, disease mechanisms and drug development by revealing the interaction network between proteins. The professional pull-down assay platform is particularly suitable for screening and identifying ligands and related proteins that interact with target proteins. This method effectively captures "prey" proteins that bind to a specific "bait" protein, helping to reveal key nodes in protein networks. The combination of pull-down assay service with Mass Spectrometry (MS) enhances the detection depth and accuracy of protein-protein interactions, providing a powerful tool for uncovering the dynamic and intricate protein interactions that drive cellular processes.

Pull-Down based Protein Analysis Service at MtoZ Biolabs

MtoZ Biolabs has extensive experience in pull-down based protein analysis service for target protein discovery. Characterized by strict quality control and short turnaround, we can provide you with customized one-stop solutions covering every step of your project from gene synthesis to data reporting:

1. Gene Synthesis and Expression Plasmid Construction

MtoZ Biolabs offers complete gene synthesis and plasmid construction services, ranging from gene sequence design to the generation of expression vectors. Through efficient DNA synthesis and optimized plasmid design, the target gene is accurately incorporated into the expression vector. This process includes gene optimization to enhance expression efficiency and accurate plasmid construction, ensuring the success of downstream protein expression and purification.

2. Expression, Purification, and Immobilization of Bait Proteins

To capture interacting proteins, we first express and purify bait proteins, then immobilize them on a suitable support matrix. By optimizing expression conditions, we ensure high-yield and bioactive bait proteins in either prokaryotic or eukaryotic systems. The purified proteins are immobilized on the support matrix via chemical coupling or physical adsorption, ensuring high-affinity interactions with target proteins in the following capture stages.

3. Expression and Purification of Prey Proteins

Prey proteins, like bait proteins, undergo careful design and expression to ensure precise interaction. These proteins are expressed in appropriate systems and purified to remove contaminants, yielding high-purity proteins that improve the specificity and sensitivity of subsequent interaction analyses.

4. Incubation, Washing, and Elution of Interacting Proteins

Under optimal binding conditions, the bait protein is incubated with prey proteins to facilitate interaction. Sequential washing steps remove non-specifically bound proteins, and finally, interacting proteins are eluted under mild conditions, yielding high-purity protein complexes for further analysis.

5. Protein Interaction Validation via SDS-PAGE and Western Blotting

MtoZ Biolabs employs SDS-PAGE and Western Blotting to verify protein interactions. SDS-PAGE provides a clear molecular weight profile, and Western Blotting confirms the specificity of interacting proteins through targeted antibody detection, providing preliminary validation for subsequent mass spectrometric analysis.

6. LC-MS Identification of Interacting Proteins

Liquid chromatography coupled with mass spectrometry (LC-MS) is employed to characterize interacting proteins. This high-resolution technique enables quantitative and qualitative analysis of complex protein mixtures, identifying even low-abundance proteins and offering detailed compositional insights into interacting proteins.

7. Data Analysis and Comprehensive Reporting

Following experimentation, MtoZ Biolabs provides in-depth data analysis and detailed project reports encompassing protein identification, interaction analysis, and functional annotation. This comprehensive report enables clients to interpret the biological relevance of protein interactions and informs experimental planning for further research and development.

Applications

The following application scenarios illustrate the significance of pull-down based protein analysis service in advancing both basic research and clinical studies. Facilitating the detailed elucidation of biological mechanisms, the identification of novel therapeutic targets, and the acceleration of drug discovery and development.

1. New Drug Target Discovery

In the drug discovery and development process, Pull-Down experiments enable precise identification of target proteins within protein complexes, supporting research and development teams in validating prospective drug targets within key signaling pathways study.

2. Protein Interaction Networks Study

The complex networks of protein interactions are central to the regulation of numerous physiological processes in organisms. Pull-Down experiments enable researchers to identify critical node proteins and characterize their interactions, offering profound insights into underlying physiological mechanisms.

3. Discovery of Disease Biomarkers

In disease biomarker research, identifying protein interactions that are functionally related to specific diseases can advance clinical detection and diagnostic approaches. By capturing these aberrant interacting proteins through Pull-Down experiments followed by mass spectrometry analysis, it is possible to screen candidate biomarkers of diagnostic significance.

4. Antibody-Antigen Binding Studies

The pull-down assay service effectively models antibody binding with target antigens under in vitro conditions, facilitating the validation of antibody specificity and affinity. This approach expedites the antibody drug development process by providing reliable in vitro interaction data.

Why Choose MtoZ Biolabs?

Our primary advantages include:

1. Advance Analysis Platform

MtoZ Biolabs's advanced pull-down assay platform, guaranteeing reliable, fast, and highly accurate analysis service.

2. One-Time-Charge

Our pricing is transparent, no hidden fees or additional costs.

3. High-Data-Quality

Deep data coverage with strict data quality control. AI-powered bioinformatics platform integrate all pull-down based protein analysis service data providing clients with a comprehensive data report.